show Abstracthide AbstractThe nearly neutral theory of molecular evolution predicts that the efficacy of both positive and purifying selection is a function of the long term effective population size (Ne) of a species. Under this theory, the efficacy of natural selection should increase with Ne. Here, we tested this simple prediction by surveying ~1.5-1.8 Mb of protein coding sequence in the two subspecies of the European rabbit (O. c. algirus and O. c. cuniculus), a mammal species characterized by high levels of nucleotide diversity and Ne estimates for each subspecies on the order of 1X106. When the segregation of slightly deleterious mutations and demographic effects were taken into account, we inferred that >60% of amino acid substitutions on the autosomes were driven to fixation by positive selection. Moreover, we inferred that a small fraction of new amino acid mutations (< 4%) are effectively neutral (defined as 0 < Nes < 1), and that this fraction was negatively correlated with a gene’s expression level. Consistent with models of recurrent adaptive evolution, we detected a negative correlation between levels of synonymous sites polymorphism and the rate of protein evolution, although the correlation was weak and non-significant. No systematic X-chromosome-autosomes difference was found in the efficacy of selection. For example, the proportion of adaptive substitutions was significantly higher on the X-chromosome compared to the autosomes in O. c. algirus, but not in O. c. cuniculus. Our findings support widespread positive and purifying selection in rabbits, and add to a growing list of examples suggesting that differences in Ne among taxa play a substantial role in determining rates and patterns of protein evolution.